Table 2.

Equine primer and probe sequences used for gene expression analyses.

Gene	Primer and probe sequences
18S, 18 small ribonucleic acid	Forward, 5′- GCCGCTAGAGGTGAAATTCT-3′
	Reverse, 5′- TCGGAACTACGACGGTATCT−3′
	Probe, 5′- AAGACGGACCAGAGCGAAAGCAT-3′
RUNX2, runt-related transcription factor 2	Forward, 5′-GAACCCAGAAGGCACAGACA-3′
	Reverse, 5′-GGCTCAGGTAGGAGGGGTAA-3′
	Probe, 5′-ATTTAGGGCGCATTCCTCATCCCA-3′
ALP, alkaline phosphatase	Forward, 5′-CGGGACTGGTACTCGGAC-3′
	Reverse, 5′-CATGTACTTCCGGCCGCC-3′
	Probe, 5′-AAGGACATTGCCTACCAGCTCGTG-3′
OC, osteocalcin	Forward, 5′-GTGCAGAGTCTGGCAGAGGT-3′
	Reverse, 5′-CCAGCCAATGATCCAGGTAG-3′
	Probe, 5′-AATCTCTTCACCACCTCACTGCCC-3′
PPARγ, peroxisome proliferator-activated receptor	Forward, 5′-TCTCATTGACCCAGAAAGCGA−3'
	Reverse, 5′-CCACTTTGATCGCACTTTGGTA-3′
	Probe, 5′-TGCAAGAGCTGATCCCATGGTTGA-3′
ACAN, aggrecan	Forward, 5′-GAGGAGATGGAGGGTGAGGT−3′
	Reverse, 5′-GATGGTGATGTCCTCCTCGC-3′
	Probe, 5′-TTCACCTGTGTAGCAGATGGCGTC-3′
COL2b, type II collagen	Forward, 5′-GCTACACTCAAGTCCCTCAAC-3′
	Reverse, 5′-ATCCAGTAGTCTCCGCTCTT-3′
	Probe, 5′-ACCTGAAACTCTGCCACCCTGAAT-3′
SOX9, SRY-box 9	Forward, 5′-CTGGAGACTGCTGAACGAGA-3′
	Reverse, 5′-GAGATGTGTGTCTGCTCCGT−3′
	Probe, 5′-AGAAGGACCACCCGGACTACAAGTA-3′