Figure 1.

Changes in the steady-state transcript levels of the plastid ndhB and ndhF genes in response to different light treatments. Primary leaves from young (7-d-old) and mature-senescent (14-d-old) plants were incubated for 20 h at 23°C in darkness (D), GL (100 μmol photon m⁻² s⁻¹), or PhL (300 μmol photon m⁻² s⁻¹). Total RNA was isolated from barley leaves, separated by agarose electrophoresis (12.5 μg each), blotted on a nylon membrane, and hybridized with ndhB or ndhF probes or stained with methylene blue (for rRNAs) as described in “Materials and Methods.”