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. 2019 Jun 6;7:95. doi: 10.3389/fcell.2019.00095

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Copyright © 2019 McGinn, Galicia, Leoni, Partington, Mitchell and Stenkamp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparative birthdating of regenerated PKCα+ bipolar (BP) neurons and HuC/D+ amacrine and ganglion cells following a chemical lesion selective to the inner retina. (A) Timeline and experimental design; DPI, days post-injury. (B) Some regenerated PKCα+ (green) BPs incorporated BrdU (magenta) (6–9 DPI). Higher magnification insets of boxed region show PKCα and DAPI, PKCα, and BrdU, and all channels merged. Arrows indicate BrdU+ PKCα+ BP cell. (C) Some regenerated PKCα+ BPs incorporated EdU (red) (9–13 DPI). Higher magnification insets corresponding to boxed region show PKCα and DAPI, PKCα and EdU, and all channels merged. Arrow indicates EdU+ PKCα+ BP cell. (D) Percentage of PKCα+ cells that incorporate BrdU and EdU at the indicated timeframes (means ± s.d.; n = 3). (E) Some regenerated HuC/D+ (green) neurons incorporated BrdU (magenta) (6–9 DPI). Higher magnification insets corresponding to boxed region show HuC/D and DAPI, HuC/D and BrdU, and all channels merged. Arrow indicates BrdU+ HuC/D+ cell. (F) Some regenerated HuC/D+ BPs incorporated EdU (red) (9–13 DPI). Higher magnification insets corresponding to boxed region show HuC/D and DAPI, HuC/D and EdU, and all channels merged. Arrow indicates EdU+ HuC/D+ cell. (G) Percentage of HuC/D+ neurons that incorporate BrdU and EdU at the indicated timeframes (means ± s.d.) are similar to the percentages of PKCα+ BPs that incorporated these labels. Scale bar in B (applies to B,C,E,F) = 50 μm. Scale bar in insets (applies to all insets) = 10 μm.