Figure 3.

Galleries of traced nyx::mYFP+ retinal bipolar (BP) neurons. Neurons were traced in Simple Neurite Tracer (SNT), dendrites (green), cell bodies (white), axons (red) were pseudocolored, and then merged with images of the DAPI-stained (blue) nuclei to visualize retinal layers; merged images were then resliced to show the orthogonal views, with preservation of alignment of the traced cells and DAPI stained nuclei. For some BPs the cell body was not traced or only minimally traced due to limitations of SNT. Top row: BPs sampled from control retinas included some that were also shown in McGinn et al. (2018). Second row: BPs sampled at 13 days post-injury (13 DPI) vary in appearance, with some displaying long, wandering axons, and others displaying simple apical processes but no dendritic trees, and others presenting an apparently normal morphology. Third row: BPs sampled at 17 DPI also vary in appearance, with unusual dendritic tree structures or only simple apical processes, and others appearing normal. Bottom row: BPs sampled at 21 DPI show morphologies that more closely resembled those of control retinas. Scale bar = 10 μm.