Table 2.

Oligosaccharide sequences identified by ultra high pressure liquid chromatography-mass spectrometry untargeted in-source fragmentation (UHPLC-MS²) from ULVAN-01 (sequences 1–4 [48]) and ULVAN-02 (sequences 5–8).

Oligosaccharide Sequence	Ion (m/z)			Ionization	Molecular Formula	Symbolic Representation of Oligosaccharide Fragments (a) (CFG Nomenclature (b)) Rha, GlcA (or IdoA) (c) Glc (or Gal) (c), Xyl S = Sulfate Group
	Experimental Value	Predicted Value	Resolution (ppm)
1	1561.3492	1561.3516	1.54	[M − H]−	C54H81O50S
2	1561.3492	1561.3516	1.54	[M − H]−	C54H81O50S
3	1723.4083	1723.4045	2.20	[M − H]−	C60H91O55S
4	1839.4554	1839.4518	1.95	[M − H]−	C65H99O58S
5	401.0385	401.0390	1.25	[M − H − H2O]−	C12H17O13S
6	502.9772	502.9777	0.99	[M − 2H − H2O + Na]−	C12H16O16S2Na
7	604.9165	604.9165	0.00	[M − 3H − H2O + 2Na]−	C12H15O19S3Na2
8	706.8561	706.8553	1.13	[M − 4H − H2O + 3Na]−	C12H14O22S4Na3

^(a) Identified ions correspond to oligosaccharide fragments derived from untargeted fragmentation in the ionization source of the mass spectrometer; ^(b) Consortium for Functional Glycomics. Symbolic representations were obtained using Glycoworkbench software [52]; ^(c) GlcA and IdoA residues, as well as Glc and Gal, are isomers and therefore cannot be distinguished by MS analysis: symbols used for GlcA et Glc can thus correspond to IdoA and Gal, respectively, two monosaccharides that can be found in ulvans but in smaller proportions.