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. 2019 May 21;8(5):482. doi: 10.3390/cells8050482

Figure 1.

Figure 1

Apoptosis, necrosis, and autophagy in bone marrow-derived mesenchymal stem cells from patients with acute myeloid leukemia after 24 h 7-KC treatment. A: Cells were treated with or without Z-VAD-FMK for 3 h followed by incubation with 7-KC for 24 h. Cytotoxicity (apoptosis) was evaluated by Hoechst 33342/propidium iodide staining. B: Percentage of apoptotic cells determined by the externalization of phosphatidylserine. C: Cells were treated with or without necrostatin-1 for 3 h followed by incubation with 7-KC for 24 h. Cytotoxicity (necrosis) was evaluated by Hoechst 33342/propidium iodide staining. D: Percentage of cells with necrosis determined by propidium iodide. E: Cells were treated with or without 3-MA for 2 h followed by incubation with 7-KC for 24 h. Cytotoxicity (autophagy) was evaluated by Hoechst 33342/propidium iodide staining. F: Percentage of cells with autophagy as evaluated by Premo Autophagy Sensor. CD: chloroquine diphosphate. G–K: Representative figures of LC3B-green fluorescent protein (GFP) chimera sensing of autophagy (G: 25 µM 7-KC; H: 50 µM 7-KC; I: 100 µM 7-KC; J: CD; and K: control). Data are mean ± SEM from three independent experiments in duplicate. * p < 0.05, ** p < 0.01, *** p <0.001. Scale bar, 100 µM.