Activation of cell death in in Jurkat T-lymphocytes. (A,B) Whole cell patch clamp currents activated by ionomycin (Iono, 1 µM) in Jurkat T lymphocytes, and corresponding current/voltage relationships. (C) Activation of whole cell currents by tert-butyl hydroperoxide (tBHP; 50 μM/6 h) and inhibition of currents by 5Cl. (D,E) Activation of cell death by RSL3 + erastin and inhibition by Figure 1. (Fer-1, 5 µM) or tannic acid (TA, 10 µM). Mean ± SEM (number of experiments). * significant activation by Iono (p < 0.05, paired t-test). # significant difference when compared with scrambled, control, or 5Cl, respectively (p < 0.05, unpaired t-test). $ significant inhibition (p < 0.05, ANOVA).