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. 2019 May 5;11(5):625. doi: 10.3390/cancers11050625

Figure 4.

Figure 4

Phospholipid scrambling by TMEM16F and cooperativity with TMEM16A. (A) Flow cytometry in HEK293 cells expressing TMEM16F, TMEM16A, TMEM16A in the presence of siRNA for TMEM16F, or cells transfected with empty plasmid (mock) or siRNA for TMEM16F. 4-quadrant dot blot graphs showing 7-AAD positivity on y-axis and annexin V positivity on x-axis. Phospholipid scrambling (PS; annexin V positivity) was induced by stimulation of the cells with 1 µM Iono; 10 min). (B) Summary of % annexin V positive cells before and after exposure to ionomycin. Mean ± SEM (number of experiments). # indicates significant difference when compared to control (p < 0.05, unpaired t-test).