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Amplification curves by absolute quantification. The y-axis shows # copies/well and the axis x-axis shows the cDNA concentration used in ddPCR. (a) Copy number transcript by well of ACTB, GAPDH, RPL4 and RPS18. ACTB was the most highly expressed housekeeping gene. (b) Copy number transcript by well of PSMB6, PGGT1B, UBQLN2 and UQCR2. UQCR2 was the most highly expressed following PSMB6.
