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. 2019 May 21;11(5):701. doi: 10.3390/cancers11050701

Figure 5.

Figure 5

SW480-derived TG2 drives fibroblast-mediated structural alterations in co-cultures. Sirius red staining of fibroblast/SW480 co-cultures showed regions of dense collagen at the CRC/stroma boundary with untreated SW480 cells (A, white arrows) and thinning regions at this boundary once SW480 cells were treated with TG2 siRNA (B, white arrows). Collagen fibre thickness was significantly lower once SW480 cells were introduced into cultures, compared to gels cast with fibroblasts alone (C, n = 27). When treated with TG2 siRNA, the reduced fibre thickness induced by SW480 cells was partially reversed (C, n = 27). SW480 cells also resulted in less straight fibres compared to gels cast with fibroblasts alone (D, n = 27), but TG2 siRNA treatment of the SW480 cells had no effect. Significant differences in fibre length were not observed between gels cast with fibroblasts alone, SW480 cells, or with SW480 cells treated with TG2 siRNA (E, n =27). *** p < 0.001.