Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 10;8(5):436. doi: 10.3390/cells8050436

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

MG-132 attenuated fluoride-induced apoptosis in LS8 cells. LS8 cells were treated with MG-132 (0.5–1.0 μM) for 2 h prior to NaF (5 mM) treatment for 24 h. (a) The Bcl-2/Bax mRNA ratio was quantified by qPCR. NaF significantly decreased the Bcl-2/Bax mRNA ratio (** p < 0.01). (b) MG-132 (1 μM) significantly increased the Bcl-2/Bax ratio compared to NaF treatment alone (* p < 0.05). Data are presented as means ± SD. (c) γH2AX (15 kDa) and cleaved-caspase-3 (17 kDa) were detected by Western blots. MG-132 inhibited fluoride-induced γH2AX protein expression and inhibited caspase-3 cleavage. The numbers show relative expression normalized by the loading control β-actin (44 kDa). Statistical analysis of relative protein expression of cleaved-caspase-3 and γH2AX are shown in supplementary Figure S6.