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. 2019 May 25;11(5):727. doi: 10.3390/cancers11050727

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Dose-dependent development of dermal fibrosis. Representative images of ear sections on day 92 after a 4-fraction course using different doses per fraction: sham (A), 5 Gy (B), 10 Gy (C), 20 Gy (D), 30 Gy (E). Sections are stained with Sirius red for demonstration of collagenous connective tissue (dark red color). Important anatomical structures are indicated in E. Ear thickness and connective tissue deposition in the dermal layers of the ear skin increase with the radiation dose. Paraffin sections. Scale bar: 100 µm.