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. 2019 May 25;11(5):727. doi: 10.3390/cancers11050727

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical detection of TGFβ1 (brown color) in mouse ear sections on day 92 following hypofractionation with different doses per fraction: sham (A), 5 Gy (B), 10 Gy (C), 20 Gy (D), 30 Gy (E). Positive endothelial and intravascular inflammatory cells are indicated by arrowheads, epidermal immunoreactivity by closed arrows, and immunoreactivity of dermal cells by open arrows. Paraffin sections of murine ears are shown. Chromogenic substrate: diaminobenzidine (DAB), nuclear counterstain: hematoxylin. Scale bar: 200 µm (for zoom-in image 20 µm).