Figure 13.

Anatomical landmarks used for definition of the ROI in transversal ear sections. (A) Histological section of the pinna. The radiated region of the pinna is indicated by a black box. The dotted line in (B) indicates the orientation of the section through the radiated area of the pinna. (C) ROI sampled for histological analysis and morphometric analysis (dotted rectangle in A). The ROI contains at least two section profiles of ear veins (arrowheads) and skeletal musculature. The dotted line marks the interface of the ear cartilage, used to measure the length l of the ROI (i.e., the section width). (D) Detail enlargement of the image shown in C, demonstrating relevant histological structures. Formalin-fixed paraffin embedded sections, H&E staining. Scale bar = 1 mm in A and C, and 100 µm in D.