Table 1.
Composition of the used solutions with concentrations in mM and an assigned solution number used throughout the manuscript.
| Solution | PBS | ECS HEPES | ECS HEPES − Glucose | pbECS | pbECS + HEPES | ECS MOPS | pbECS − Ca2+ | pbECS − Ca2+ 3 × Buffered | |
|---|---|---|---|---|---|---|---|---|---|
| (M) | #1 | #2 | #3 | #4 | #5 | #6 | #7 | #8 | |
| NaCl | 137.9 | 145.0 | 145.0 | 133.0 | 133.0 | 145.0 | 133.0 | 108.8 | |
| KCl | 2.5 | 5.0 | 5.0 | 3.5 | 3.5 | 5.0 | 3.5 | 0.6 | |
| Glucose | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | |||
| KH2PO4 | 2.5 | 1.5 | 1.5 | 1.5 | 4.4 | ||||
| Na2HPO4 | 7.0 | 8.1 | 8.1 | 8.1 | 24.2 | ||||
| CaCl2 | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 | |||
| MgCl2 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | |
| EGTA | 10.0 | 10.0 | |||||||
| HEPES | 10.0 | 10.0 | 10.0 | ||||||
| MOPS | 10.0 | ||||||||
EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid), MOPS (3-(N-morpholino)propanesulfonic acid).