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. 2019 May 5;8(5):415. doi: 10.3390/cells8050415

Figure 4.

Figure 4

KLK12 mediated regulation of autophagy in M. bovis C68004 infected murine BMDMs implicates AMPK/TSC2/mTOR signaling pathway. Murine BMDMs were transfected with KLK12 siRNA and negative control siRNA (20uM). After 48 h of transfection, murine BMDMs were infected with M. bovis C68004 at a MOI of 10 and incubated for indicated time periods (6 and 24 h). Then, expression of phosphorylated AMPK, TSC2 and mTOR was determined in KLK12 siRNA and negative control siRNA transfected BMDMs by the Western Blot analysis. Expression of all the proteins was normalized to β-Actin expression. Data represent the mean ± SD of three independent experiments (* p < 0.05; ** p < 0.01).