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. 2019 May 5;8(5):415. doi: 10.3390/cells8050415

Figure 6.

Figure 6

KLK12 regulates cytokines expression in the M. bovis C68004 infected murine BMDMs. (A,B) Murine BMDMs were transfected with KLK12 siRNA and negative control siRNA (20 uM). After 48 h of transfection, murine BMDMs were infected with M. bovis C68004 at a MOI of 10 and incubated for 6 (A) and 24 h (B); expression of IL-1β, IL-6, IL-12, TNF-α, IL-10 and IFN-β was determined by qRT-PCR and β-Actin was taken as internal control; (C,D) murine BMDMs were transfected with KLK12 siRNA and negative control siRNA and infected with M. bovis C68004 as stated above. Then, expression of IL-12, IL-10 and IFN-β were measured in cell supernatants by using ELISA kit after 6 (C) and 24 h (D) of incubation. Data represent the mean ± SD of three independent experiments (* p < 0.05; ** p < 0.01; ns, non-significant).