Figure 2.

Effect of DCA on vital parameters and cell migration capacity, (A) measurement of early and late apoptotic and necrotic cells was performed by flow-cytometry as described in Material and Methods, cells were treated with 4 and 10 mM DCA for 24 h (left panel) and 48 h (right panel), data are expressed as percentage of total events analyzed and are the mean ± SEM of at least three independent experiments (* p < 0.05 vs. CTRL, ** p < 0.01 vs. CTRL); (B) expression of caspase 3, (upper panel) representative western blotting of total protein lysate, (lower panel) densitometric analysis (normalized to β-actin) of the uncleaved caspase 3 band, the bars are means ± SEM of three independent experiments under each condition; NS, not significant.