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. 2019 May 5;11(5):627. doi: 10.3390/cancers11050627

Figure 1.

Figure 1

Figure 1

DFX117 significantly induces growth inhibition of non-small cell lung cancer cells. (A) The proliferation of four lung cancer cell lines (A549, NCI-H1975, PC9 and NCI-H1993) were significantly inhibited by DFX117. (B) DFX117 showed few effects on cell growth of normal cell lines MRC-5 at higher concentration. (C) The chemical structure of DFX117 and the IC50 values of DFX117 against non-small cell lung cancer cells or normal cell lines. (D) Colony formation assays were performed in the A549 and NCI-H1975 cells treated with various concentrations of DFX117. 500 cells were plated in 6 wells plates and incubated for 12 h before treatment with DMSO or DFX117 at indicated concentration for 72 h. Then cells were then washed once with PBS and continue to culture with full-growth medium for 10 days, change the culture medium to fresh ones every three days. Cells were stained with 0.1% crystal violet after fixing with 100% methanol. (E) Representative images of the colony formation assay are depicted and compared with HS-173, a PI3Kα inhibitor. * p < 0.05 or ** p < 0.01 was considered statistically significant compared with the corresponding control values.