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. 2019 May 5;11(5):627. doi: 10.3390/cancers11050627

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A1 — DFX117 decreased the mRNA levels of PIK3CA in A549 and NCI-H1975 xenograft model. (A) DFX117 decreased the mRNA levels of PIK3CA in A549 xenograft in athymic mice. (B) DFX117 decreased the mRNA levels of PIK3CA in H1975 xenograft in athymic mice. The total RNA of the tumor tissue was isolated with TRI reagent (Invitrogen, Grand Island, NY, USA). mRNA expression of PIK3CA were detected by real-time PCR using β-actin as a reference gene. Data are presented as the mean fold changes ± SD of three independent experiments. * p < 0.05, ** p < 0.01.