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. 2019 May 14;11(5):672. doi: 10.3390/cancers11050672

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Interplay of TLR-6/-9 signaling in THP-1 cells following treatment with ODN2006 and M362. Protein expression and densitometry analysis of TLR-6/-9 proteins and their downstream signaling factors, namely IRAK4, IRF7, IRF5, and IRF3, following treatment with ODN2006 (A,B), ODN M362 (C,D), and ODN2216 (E,F). As TLR-6 siRNA treatment induced decreased expression of TLR-9 (A–D), we checked if this could be due to off-target effects. However, as shown in (G–J), three different TLR-6 siRNAs induced the decreased expression of TLR-9, suggesting interplay between TLR-6 and -9 expression. All experiments were performed in four independent replicates, and data are expressed as mean ± SD, * p < 0.05 compared with the MamA2.1 peptide treatment alone.

Interplay of TLR-6/-9 signaling in THP-1 cells following treatment with ODN2006 and M362. Protein expression and densitometry analysis of TLR-6/-9 proteins and their downstream signaling factors, namely IRAK4, IRF7, IRF5, and IRF3, following treatment with ODN2006 (A,B), ODN M362 (C,D), and ODN2216 (E,F). As TLR-6 siRNA treatment induced decreased expression of TLR-9 (A–D), we checked if this could be due to off-target effects. However, as shown in (G–J), three different TLR-6 siRNAs induced the decreased expression of TLR-9, suggesting interplay between TLR-6 and -9 expression. All experiments were performed in four independent replicates, and data are expressed as mean ± SD, * p < 0.05 compared with the MamA2.1 peptide treatment alone.