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Schematic drawing of the NIR fluorescence confocal imaging system, including the fiber-based multi-color laser excitation and multi-channel fluorescence emission collection system based on photomultiplier tubes (PMT), control system, signal amplification, data acquisition, and real-time image reconstruction; the gain settings of the PMTs were adjusted along the Z-axis for depth imaging. Note: LP filter: long-pass filter, DM: dichroic mirror.
