Table 2.
13C and 1H NMR data of pyrrospirone J (10, in DMSO-d6).
| No. | δC, Type | δH (J in Hz) | 1H-1H COSY | HMBC |
|---|---|---|---|---|
| 1 | 47.3, CH2 | βH: 0.81, t (12.3); αH: 1.80, m |
H-2, αH-2; H-2, βH-2 |
C-26, C-28 |
| 2 | 27.4, CH | 1.82, m | H-1, H-3, H3-26 | C-6 |
| 3 | 45.5, CH2 | βH: 0.51, q (12.1); αH: 1.75, m |
H-2, αH, H-4 H-2, βH, H-4 |
C-2, C-27 |
| 4 | 26.8, CH | 1.78, m | H-3, H-5, H3-27 | C-2 |
| 5 | 59.5, CH | 1.22, dd (11.6, 8.8) | H-4, H-9 | C-4, C-6, C-28 |
| 6 | 42.5, C | – | ||
| 7 | 49.9, CH | 1.55, d (14.3) | H-8 | C-1, C-5, C-6, C-8, C-13, C-28 |
| 8 | 39.2, CH | 2.79, m | H-7, H-9, H-13 | C-7, C-9, C-10, C-13, C-14 |
| 9 | 85.0, CH | 4.82, dd (8.6, 7.0) | H-5, H-8 | C-5, C-6, C-13, C-23 |
| 10 | 58.8, C | – | ||
| 11 | 63.5, CH | 2.46, s | C-10, C-12, C-13, C-29, C-30 | |
| 12 | 81.3, C | – | ||
| 13 | 44.5, CH | 3.21, d (8.0) | C-8, C-9, C-11, C-12, C-14, C-30 | |
| 14 | 180.9, C | – | ||
| 15 | 79.7, C | – | ||
| 16 | 172.1, C | – | ||
| 17 | 86.2, C | – | ||
| 18 | 40.9, CH2 | βH: 1.99, d (12.1); αH: 2.45, d (12.1) |
αH-18; βH-18 |
C-14, C-15, C-16, C-17, C-19 |
| 19 | 45.0, CH2 | βH: 2.99, d (14.5); αH: 2.69, d (14.5) |
αH-19; βH-19 |
C-17, C-18, C-20, C-21, C-25 |
| 20 | 129.4, C | – | ||
| 21 | 133.3, CH | 6.92, dd (8.3, 2.1) | H-22 | C-19, C-23, C-25 |
| 22 | 124.4, CH | 6.82, dd (8.3, 2.7) | H-21 | C-20, C-23, C-24 |
| 23 | 158.7, C | – | ||
| 24 | 119.7, CH | 6.94, dd (8.8, 2.7) | H-25 | C-20, C-22, C-23 |
| 25 | 130.6, CH | 6.78, dd (8.8, 2.1) | H-24 | C-19, C-21, C-23 |
| 26 | 22.7, CH3 | 0.86, d (6.1) | H-2 | C-1, C-2, C-3 |
| 27 | 19.7, CH3 | 1.02, d (6.2) | H-4 | C-3, C-4, C-5 |
| 28 | 15.2, CH3 | 1.05, s | C-1, C-5, C-6, C-7 | |
| 29 | 21.0, CH3 | 1.20, s | C-7, C-10, C-11 | |
| 30 | 26.0, CH3 | 1.59, s | C-11, C-12, C-13 | |
| OH-17 | – | 6.24, s | C-17, C-18, C-19 | |
| NH-16 | – | 8.76, s | C-15, C-16, C-17, C-18 |