Table 3.
Specificity codes inferred from the protein sequence of the adenylation domain of McyC according to [31] or NRPS predictor2 [32,33]. Conserved amino acids are highlighted in light blue and variable amino acids are highlighted in dark green. Homo-amino acids are highlighted in red.
| Organism | Strain | McyC | P * | % | Aa # | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 235 | 236 | 239 | 278 | 299 | 301 | 322 | 330 | 331 | 517 | |||||
| Phormidium sp. | LP904c | D | A | L | F | F | G | L | V | D | K | Leu | 100 | Arg, Hty, Hph, Trp |
| Phormidium sp. | DVL1003c | D | A | L | C | F | G | L | V | D | K | Leu | 100 | Arg, Hty, Hph, Trp |
| Planktothrix agardhii | CYA 126/8 | D | P | W | G | F | G | L | V | D | K | Gln | 70 | Arg |
| Planktothrix agardhii | 213 | D | P | W | C | F | G | L | V | D | K | Gln | 70 | Arg |
| Planktothrix agardhii | NIVA-CYA 56/3 | D | P | W | G | F | G | L | V | D | K | Gln | 70 | Arg |
| Planktothrix prolifica | NIVA-CYA 98 | D | P | W | G | F | G | L | V | D | K | Gln | 70 | Arg |
| Planktothrix rubescens | NIVA-CYA 407 | D | P | W | G | F | G | L | V | D | K | Gln | 70 | Arg |
| Dolichospermum sp. ∞ | 90 | D | V | W | C | F | G | L | V | D | K | Ser | 80 | Arg |
| Dolichospermum flos-aquae ∞ | 18B6 | D | V | W | S | F | G | L | V | D | K | Ser | 80 | Arg |
| Dolichospermum lemmermannii ∞ | 66 A | D | V | W | S | F | G | L | V | D | K | Ser | 80 | Arg |
| Nostoc sp. | 152 | D | V | W | N | F | G | F | I | D | K | Gln | 70 | Arg, Har |
| Nostoc sp. | IO-102-I | D | V | W | N | F | G | F | V | D | K | Glu | 70 | Arg |
| Fischerella sp. | PCC9339 | D | V | W | F | F | G | L | V | D | - | Ser | 70 | Arg |
| Hapalosiphon hibernicus | BZ-3-1 | D | V | W | F | F | G | L | V | D | K | Ser | 80 | Ala, Leu, Val |
| Microcystis aeruginosa | PCC 7806 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Arg |
| Microcystis aeruginosa | FCY-28 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Ni |
| Microcystis aeruginosa | FCY-26 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Ni |
| Microcystis aeruginosa | UV027 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Arg |
| Microcystis aeruginosa | K139 | D | V | W | T | I | G | A | V | E | K | Arg | 100 | Arg |
| Microcystis aeruginosa | DIANCHI905 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Ni |
| Microcystis aeruginosa | PCC 9807 | D | V | W | T | I | G | I | V | D | K | Arg | 90 | Ni |
| Microcystis aeruginosa | PCC 7941 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Ni |
| Microcystis aeruginosa | PCC 9443 | D | V | W | T | I | G | I | V | D | K | Arg | 90 | Ni |
| Microcystis aeruginosa | NIES-843 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Arg |
| Microcystis aeruginosa | SPC777 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Arg |
| Microcystis viridis | NIES 102 | D | V | W | T | I | G | A | V | D | K | Arg | 100 | Arg |
P * prediction by NRPSpredictor2; Aa # amino acid detected by LC-MS (Supplementary Table S5); X. MC contains an unknown amino acid or the overall amino acid content is not known; Ni. No information. ∞ Planktonic Anabaena have recently been taxonomically assigned to the genus Dolichospermum.