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. 2019 May 18;10(5):378. doi: 10.3390/genes10050378

Figure 1.

Figure 1

Sustained expression of D-GADD45 induces JNK-dependent cell death. (A–D) TUNEL assay of wing discs labeling apoptotic cells after sustained expression of each construct in the posterior (hedgehog, hh) compartment. Apoptotic cells (red), posterior compartment (green) and DNA (white). Scale bar: 50 μm. (E) Histogram showing the apoptotic index for each genotype in the green fluorescent protein (GFP)-positive and GFP-negative compartments. Error bars indicate standard deviation. N ≥ 4 discs for each genotype. **p < 0.01. ***p < 0.001.