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. 2019 May 18;10(5):378. doi: 10.3390/genes10050378

Figure 3.

Figure 3

Transient expression of D-GADD45 is not sufficient to induce cell death. (A) Schematic representation of D-GADD45 transient activation in the posterior (engrailed, en) compartment. The affected domain is represented in orange. (B–D) TUNEL assay of wing imaginal discs labeling apoptotic cells (red), the anterior compartment (Ci: Cubitus interruptus, green) and DNA (white). N = 5 for each condition. Scale bar: 50μm. (E–G) Adult wings showing the predominant phenotype observed after transient expression of each construct in the posterior (engrailed, en) compartment. Number of wings analyzed is indicated for each condition. (H) Box plots showing the average area of adult wings. Each dot represents one wing; normal (blue) and aberrant pattern (orange).