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. 2019 May 24;17(5):305. doi: 10.3390/md17050305

Figure 6.

Figure 6

Effect of actinomycin V on the expression of E-cadherin. (A,B) MDA-MB-231 cells were treated with 0–2 nmol/L actinomycin V for 24 h then the protein expression of E-cadherin was measured by Western blot. (C) Relative expression of E-cadherin mRNA in the MDA-MB-231 cells was analyzed by real-time PCR. RNA levels are represented as fold increase relative to the level of the control (normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA level). Results were obtained from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. (D) cells were treated with 0–2 nmol/L actinomycin V for 6 h and analyzed by E-cadherin fluorescent signals. Cells were stained with anti- E-cadherin (green) and 4′,6-diamidino-2-phenylindole (DAPI, blue). Magnification ×200. E-cadherin levels were increased in the cells, consistent with the Western blot results.