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. 2019 May 27;8(5):509. doi: 10.3390/cells8050509

Figure 4.

Figure 4

Inhibition of Gli and Shh expression in (a) PDAC (PANC-1) and (b,c) human lung cancer cells (A549 and H2030, respectively) by Oxy186. Cells were treated with the compounds as indicated in DMEM or RPMI 1640 containing 5% FBS for (a) 48 or (b,c) 72 hours, then RNA was extracted and analyzed by Q-RT-PCR for the expression of Gli1 and normalized to GAPDH expression. (d) CAPAN-1 cells were treated with the compounds, as indicated, in DMEM containing 5% FBS for 72 h, then RNA was extracted and analyzed by Q-RT-PCR for the expression of Shh and normalized to GAPDH expression. Data from a representative experiment are reported as the mean of triplicate determinations ± SD (* p < 0.05 vs. control; ** p < 0.01 vs. control).