Table 2.
The total number of exonic reads in three genes expressed in human retinal tissue and three genes with low/no expression in human retina was extracted and the mean number of reads per exon calculated. Comparison of these six control genes and the values generated for NECAP1 using canine RNAseq data showed reads aligning to NECAP1 providing evidence of gene expression in the Petit Basset Griffon Vendeen (PBGV) control canine retina.
| Gene | CanFam3.1 Chromosomal Coordinates | Total Number of Exonic Reads | Mean Number of Reads Per Exon |
|---|---|---|---|
| RPGRIP1 | 15: 18316887-18387548 | 1458 | 58 |
| RHO | 20: 5632150-5637404 | 461640 | 92328 |
| GNAT1 | 20: 39129469-39133156 | 118800 | 13200 |
| ANXA1 | 1: 84744444-84763121 | 120 | 9 |
| MYH41 | 5: 34748657-34767076 | 3 | 0 |
| MYOT | 11: 25591798-25592328 | 40 | 4 |
| NECAP1 | 27: 37460607-37506942 | 749 | 94 |