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Copper chelation differently affects E-cadherin expression of colon cancer HT-29 cells bearing BRAFV600E mutation compared to HCT-116 cells carrying BRAFwt. HCT-116 and HT-29 were treated with TM (1 μM) for 72 hours and then collected to evaluate specific anti- E-cadherin and β-catenin expression by immunoblot. Anti-GAPDH was used as loading control. Densitometry was performed using ImageJ software.
