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. 2019 Apr 30;10(5):299. doi: 10.3390/mi10050299

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The scheme here illustrates the formation of GUVs using the inverted emulsion phase transfer. (a) An emulsion is formed by mixing the aqueous solution and the lipid mixture. The emulsion is stabilised due to the presence of the lipid molecule that act as surfactants. (b) The emulsion formed is then transferred to another tube containing an aqueous phase. The emulsion is then added to this tube and the lipids form a monolayer at the interface. (c) The water in oil (W/O) droplets pass through the interface and pick up the second layer of lipid due to gravity or a centrifugal force. This subsequently forms a lipid vesicle with a bilayer. Reproduced from reference [81]. Copyright 2003 American Chemical Society.