| ddH2O | 3.6 µL |
| iTaqSYBR-Green master Mix (BioRad #172-5124) | 10 µL |
| Primer Mix (10 µM working concentration F+R) | 0.4 µL |
| DNA (20 µL diluted with 38 µL ddH2O) | 6.0 µL |
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