Figure 3.

CAF-mediated effects on M2-macrophage cell surface markers. Monocyte-derived macrophages in non-stimulated or stimulated conditions were incubated with irradiated and non-irradiated CAF-conditioned medium (CM) or in co-cultures (CC). Resulting expression of M2-macrophage cell surface markers CD206 and CD163 was evaluated by flow cytometry. Filled black circles indicate expression levels in control macrophage cultures (M0 and M1). Results are expressed as fold M0-controls. Data represent the mean (±SD) values from four-4 different CAF donors in conditioned medium experiments and three donors in co-culture experiments were measured independently. Non-parametric Kruskal-Wallis test and p-values were determined between control and non-irradiated CAFs, control and the two iCAF-groups individually. iCAF (irradiated CAFs); fiCAFs: fractionated-irradiated CAFs.