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. 2019 May 17;11(5):689. doi: 10.3390/cancers11050689

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CAF-induced cytokine secretion by macrophages. Monocyte-derived macrophages in non-stimulated or stimulated conditions were incubated with irradiated or non-irradiated CAFs conditioned medium (CM) or in (CAF-macrophage) co-cultures (CC). Resulting levels of cytokines secreted by macrophages were quantified by ELISA assay. Data represent the mean (±SD) values from four different CAF donors measured in duplicates. CAF-derived IL-6 was subtracted from full measurements. Non-parametric Kruskal-Wallis test and p-values were determined between control and non-irradiated CAF-CM, control vs iCAF-CM or fiCAF-CM.