Figure 5.

CAF-induced nitric oxide production by macrophages. Monocyte-derived macrophages in non-stimulated or stimulated conditions were incubated with irradiated or non-irradiated CAF-conditioned medium (CM) or in co-culture (CC), followed by analysis of expression levels of nitric oxide by macrophages, measured by DAF-2DA fluorescence assay. Black filled circles indicate expression levels in control macrophage cultures (M0 and M1). Results are expressed as fold M0-controls. Data represent the mean (±SD) values from 3 different CAF donors measured independently. Non-parametric Kruskal-Wallis test and p-values were determined between control and non-irradiated CAFs, control and (fractionated) irradiated CAFs individually.