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. 2019 May 17;11(5):689. doi: 10.3390/cancers11050689

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CAF-mediated effects on macrophage migration. For the Transwell migration assays, stimulated macrophages were initially incubated (48 h) with CM from irradiated or non-irradiated CAFs before seeding onto the insert membrane. The migration index was determined by counting transmigrated cells towards CCL19, which was added to the lower chamber. Non-parametric Kruskal-Wallis test and p-values were determined between control and non-irradiated CAF-CM, control and the two groups of iCAF-CM. Error bars indicate ± SD of five different CAF donors from triplicate determinations.