Figure 1.

Experimental setup for high energy pulsed electromagnetic field (PEMF/rPMS) treatment of tendon-like constructs. (A) Tendon derived stem progenitor cells were isolated from 3 months old female F344 rats (n = 3) and used for the generation of 3D tendon-like constructs. Four different treatment groups were established: an untreated control group, a +Il-1β treated group, a +PEMF treatment group and a +PEMF+Il-1β treatment group. After PEMF treatment regimen total RNA of the 4 treatment groups was isolated for RNASeq analysis. (B) Image of tendon-like constructs after 7 days of contraction. (C) Position of tendon-like constructs in PEMF coil during treatment.