Figure 2.

High dose sorafenib affects PD-1 expression on T cells. (A) % PD-1+ CD4+ cells (black bars) and % PD-1+CD8+ cells (grey bars) increases with high dose sorafenib (10 µM), measured by imaging flow cytometry, (B) These PD-1+ cells exhibit an increased NFAT1 nuclear localization (measured by mean similarity score), (C) These PD-1+ cells exhibit an decreased CD8+ protein expression with high dose sorafenib (10 µM), (D) the % CD4+CD25^bright cells, as a % of total CD4+ cells decreases. Experiments are n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t-test.