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. 2019 May 7;10(5):343. doi: 10.3390/genes10050343

Figure 7.

Figure 7

Comparative analysis of different sequencing platforms applied to DNA barcoding. Lengths of amplicon fragments of five barcodes regions (ITS2, psbA-trnH, ITS, matK, rbcL) for five vegetable materials in two studied formulas, and lengths of CO1 for five animal materials (including crabs) have been shown as marks. For each sequencing platform, the dotted vertical line represents the read length while the solid transverse line represents the sequencing throughput. Though the number of raw reads obtained from the platform of PacBio is less than those of the other two NGS platforms, its advantages of long-read sequencing could maintain more original and effective information.