Table 3.
Amplicons’ information in different SMRT cells and effective rate for each amplicon.
| SMRT Cell | DNA ID | Pair of Tag-Primers # | Resulting Reads | No-Blast-Hit1 Resulting Reads | Effective Rate |
|---|---|---|---|---|---|
| Cell A | HM1 | T17 | 3255 | 0 | 100.00% |
| HM2 | T18 | 3748 | 0 | 100.00% | |
| HM3 | T19 | 4058 | 0 | 100.00% | |
| SMS1 | T13 | 2527 | 0 | 100.00% | |
| SMS2 | T14 | 3095 | 0 | 100.00% | |
| SMS3 | T15 | 4443 | 0 | 100.00% | |
| SMS1 | i13 | 603 | 0 | 100.00% | |
| SMS2 | i14 | 1030 | 0 | 100.00% | |
| SMS3 | i15 | 890 | 0 | 100.00% | |
| HMA | i17 | 557 | 0 | 100.00% | |
| HMB | i18 | 890 | 0 | 100.00% | |
| HMC | i19 | 778 | 0 | 100.00% | |
| Cell B | RS1 | i5 | 279 | 0 | 100.00% |
| RS2 | i6 | 439 | 0 | 100.00% | |
| RS3 | i7 | 462 | 0 | 100.00% | |
| WW1 | i9 | 431 | 0 | 100.00% | |
| WW2 | i10 | 706 | 0 | 100.00% | |
| WW3 | i11 | 686 | 0 | 100.00% | |
| MD1 | T1 | 725 | 0 | 100.00% | |
| MD2 | T2 | 578 | 0 | 100.00% | |
| MD3 | T3 | 749 | 0 | 100.00% | |
| MD4 | T4 | 794 | 1 | 99.87% | |
| MD1 ** | i1 | 268 | 2 | 99.25% | |
| MD2 ** | i2 | 512 | 7 | 98.63% | |
| MD3 ** | i3 | 568 | 14 | 97.54% | |
| MD4 ** | i4 | 302 | 8 | 97.35% | |
| WW1 | T9 | 1276 | 0 | 100.00% | |
| WW2 | T10 | 445 | 0 | 100.00% | |
| WW3 | T11 | 1846 | 0 | 100.00% | |
| RS1 | T5 | 911 | 0 | 100.00% | |
| RS2 | T6 | 988 | 0 | 100.00% | |
| RS3 | T7 | 1617 | 0 | 100.00% | |
| Cell C | MD3 * | i3 | 291 | 0 | 100.00% |
| JL | i39 | 777 | 0 | 100.00% | |
| DK | i40 | 1396 | 0 | 100.00% | |
| FH | C9 | 2067 | 0 | 100.00% | |
| SJS1 | i23 | 1977 | 0 | 100.00% | |
| SJS2 | i22 | 3091 | 0 | 100.00% | |
| SJS3 | i21 | 1559 | 0 | 100.00% | |
| SJS1 | C1 | 898 | 2 | 99.78% | |
| SJS2 | C2 | 1041 | 0 | 100.00% | |
| SJS3 | C3 | 324 | 0 | 100.00% | |
| JHC | C6 | 1074 | 7 | 99.35% | |
| JHC | i32 | 2182 | 0 | 100.00% | |
| JHB | i31 | 1751 | 0 | 100.00% | |
| JHA | i24 | 1348 | 0 | 100.00% | |
| Total | 60,232 | 41 | 99.93% | ||
Note: no-blast-hit 1, top hits with low Max. score (≤400) or low Identities (≤90%). **, PCR cycles were 35 + 10; *, PCR cycles were 35. #,‘T’ as psbA-trnH, ‘i’ as ITS2 (the second internal transcribed spacer of nuclear ribosomal DNA), ‘C’ as CO1 (cytochrome c oxidase subunit 1).