Figure 2.

PGE₂ production and the biosynthetic enzyme expression affected by FAAH inhibitors. BV2 cells were treated with LPS and FAAH inhibitor at the indicated concentration for 18 h, and PGE₂ in the medium was measured by EIA (A). Activated BV2 cells pretreated with LPS for 18 h were incubated in the medium containing AEA (10 µM) or AA (10 µM) and PF3845 (10 µM) or URB597 (10 µM) for 30 min. PGE₂ in the medium was assayed with EIA (B). BV2 cells were incubated with LPS and PF3845 (10 µM) or URB597 (10 µM) in the presence of AEA (10 µM) or AA (10 µM) for 18 h. PGE₂ in the medium was assayed using EIA (C). Expression levels of COX-2 (D) and mPGES2 (E) were determined relative to GAPDH in BV2 cells after 8 h treatment with LPS and inhibitor at indicated concentrations. The + and – symbols indicate the presence or absence, respectively, of LPS. Data are presented as means ± S.D. (n = 3). *, **, and *** denote p < 0.05, p < 0.01, and p < 0.001, respectively.