Table 4. . Accuracy and precision for chloroquine and desethylchloroquine extracted from human EDTA whole blood, plasma and dried blood sample.

Sample type	Chloroquine					Desethylchloroquine
	Nominal concentration (ng/ml)	Measured concentration (ng/ml)	Accuracy (%)	Precision (%)		Nominal concentration (ng/ml)	Measured concentration (ng/ml)	Accuracy (%)	Precision (%)
				Between run CV (interassay)	Within run CV (intra-assay)				Between run CV (interassay)	Within run CV (intra-assay)
Whole blood (n = 5, b = 4)
LLOQ	2.56	2.63	103	7.27	5.13	3.36	3.25	96.9	6.97	4.14
QC1	7.56	7.70	102	3.46	2.74	9.40	9.70	103	3.92	2.57
QC2	91.5	94.9	104	2.11	2.78	102	106	104	2.79	1.60
QC3	1049	1093	104	2.49	1.92	1049	1090	110	2.78	1.58
ULOQ	1220	1233	101	3.40	3.59	1220	1244	102	2.49	3.76
Over curve†	2556	2681	104	2.52	2.77	2556	2650	104	5.68	2.62
DBS (n = 5, b = 6)
LLOQ	1.82	1.95	107	16.8	14.0	2.95	3.21	109	15.7	12.4
QC1	6.03	5.76	95.5	5.20	7.09	8.89	8.40	94.5	14.2	7.79
QC2	102	97.9	96.0	5.90	4.29	124	112	90.2	7.97	4.48
QC3	1334	1323	99.2	4.56	5.21	1334	1285	96.4	7.65	3.62
ULOQ	1552	1590	102	4.29	4.54	1552	1566	101	3.11	3.26
Over curve	3429	3511	102	6.97	3.41	3429	3453	101	6.30	5.25
Plasma (n = 5, b = 4)
LLOQ	1.41	1.45	103	10.1	9.64	1.41	1.43	101	6.96	12.6
QC1	4.64	4.35	93.7	5.65	6.14	4.64	4.34	93.5	3.40	5.21
QC2	56.4	55.3	98.1	5.94	3.71	56.4	53.0	94.0	3.88	3.98
QC3	524	509	97.2	6.51	3.28	524	493	94.1	4.78	4.22
ULOQ	610	597	97.9	7.53	4.02	610	598	98.0	1.31	3.51
Over curve	1932	1953	101	5.00	2.87	1932	1939	100	5.12	4.13

Over curve, that is, sample dilution integrity test.

^†Over-curve whole blood sample diluted five-times with blank plasma.

b: Number of runs; DBS: Dried blood spot; n: Number of samples in each run; QC: Quality control.