Effects of DCA on hypoglycemia-induced neuronal death. Hypoglycemia resulted in neuronal death in the subiculum (sub), CA1, and dentate gyrus (DG) of hippocampus one week after hypoglycemia. (A) Fluoro-Jade B (FJB) positive neuronal cells are observed in the CA1, subiculum, and DG one week after hypoglycemia. Scale bar = 50 μm. (B) The number of FJB (+) cells were significantly lower in the DCA-treated group than that in the control group after insult. Data are mean ± S.E.M., n = 8 from each hypoglycemia group. (C) NeuN (+) cells show live neurons in the hippocampal subiculum, CA1 and DG. Scale bar = 100 μm. (D) The number of NeuN (+) cells were considerably more increased in the DCA-treated group than in the control group after insult. Data are mean ± S.E.M., n = 3 for each sham group and hypoglycemia group. * Significantly different from vehicle treated group, p < 0.05.