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. 2019 May 10;8(5):441. doi: 10.3390/cells8050441

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DKK1 inhibition rescues RUNX2 expression. (A) MM-ASC were cultured without or with an anti-DKK1 monoclonal antibody (DKK1-Ab) or IgG as control. Whole cell lysates were extracted and analyzed by immunoblotting. Representative immunoblots of RUNX2, DKK1, p16, p21, and tubulin (loading control) are shown. (B) The reverse experiment was performed by adding either recombinant DKK1 protein or conditioned medium (CM) from MM-ASC cultures to HD-ASC cultures, incubated or not with anti-DKK1. Immunoblots are shown.