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. 2019 May 10;11(5):645. doi: 10.3390/cancers11050645

Figure 3.

Figure 3

Figure 3

Knockdown of HDAC1, 2, 3, or 8 enhances TRAIL sensitivity. DLD-1 (A) or WiDr (B) cells were transfected with siRNAs of scramble or HDAC1, 2, 3, and 8 respectively for 72 h using Lipofectamine 2000. Relative mRNA levels were normalized by α-tubulin. The values shown are mean ± SD from one of three experiments performed in triplicate. (C) After 72 h transfection, DLD-1 cells were treated with 25 ng/mL rhTRAIL 4C7 or DHER and apoptotic cells were detected using the Violet Ratiometric Membrane Asymmetry Probe. The lower right group represents living cells, the lower left group represents early apoptotic cells, and the upper left group represents late apoptotic or dead cells. Statistical analysis of DLD-1 (D) or WiDr (E) cells was shown.