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. 2019 May 10;11(5):645. doi: 10.3390/cancers11050645

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RGFP966 or PCI34051 enhances TRAIL sensitivity on 3D spheroid model. (A) 3D spheroids were constructed on DLD-1 or WiDr cells after 72 h culturing in ultra-low attachment round bottom plates. Morphology changes were observed under an inverted light microscope with 40× magnification. (B) The DLD-1 spheroids were generated and transferred to a 96-well white wall plate and caspase3/7 activity was detected after adding 5 ng/mL rhTRAIL 4C7 or DHER overnight. Luminescence were measured after 2 h incubation with caspase 3/7 reagent. p values were analyzed by one-way ANOVA in Turkey’s multiple comparison with Graphpad Prism version 7.0. * 0.01 ≤ p ≤ 0.05, ** 0.001 ≤ p ≤ 0.01.

RGFP966 or PCI34051 enhances TRAIL sensitivity on 3D spheroid model. (A) 3D spheroids were constructed on DLD-1 or WiDr cells after 72 h culturing in ultra-low attachment round bottom plates. Morphology changes were observed under an inverted light microscope with 40× magnification. (B) The DLD-1 spheroids were generated and transferred to a 96-well white wall plate and caspase3/7 activity was detected after adding 5 ng/mL rhTRAIL 4C7 or DHER overnight. Luminescence were measured after 2 h incubation with caspase 3/7 reagent. p values were analyzed by one-way ANOVA in Turkey’s multiple comparison with Graphpad Prism version 7.0. * 0.01 ≤ p ≤ 0.05, ** 0.001 ≤ p ≤ 0.01.