Figure 3.
Effects of quercetin on the formation of protein/DNA complexes between FRTL-5 cell nuclear extracts and an AP-1 (Activator protein-1) consensus oligonucleotide. Radiolabeled oligonucleotide was incubated with nuclear extracts from cells cultured in 6H5% medium (control), cells treated with 10 μM quercetin (Querc) for 48 h; cells treated with quercetin plus normal rabbit polyclonal antiserum (Querc-Cont) or antibodies against c-jun (+anti-c-jun) or c-fos (+anti-c-fos). Lane 1 contains the probe alone.