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. 2019 May 18;8(5):477. doi: 10.3390/cells8050477

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Subcellular locations and expression pattern analysis of lnc-ORA. (A) Subcellular localization of lnc-ORA by fluorescent in situ hybridization (FISH) in preadipocytes. Special FISH probes against lnc-ORA, U6, and 18s were modified by Cy3 (red); 18S is a cytoplasmic marker, and U6 is a nuclear marker. The nucleus was stained by DAPI (blue). (B) Expression pattern of lnc-ORA during preadipocytes proliferation. (C) Expression levels of lnc-ORA after inducing adipocytes differentiation. Data are expressed as the mean ± SEM (n = 3).