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. 2019 May 18;8(5):477. doi: 10.3390/cells8050477

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Knockdown of lnc-ORA inhibits adipocytes differentiation. (A) BODIPY staining of adipocytes after induction for 6 d. (B) Knockdown efficiency of lnc-ORA after adipocyte transfection for 2 d and 4 d. (C–E) mRNA levels of adipocytes markers, including PPARγ, FASN, and FABP4, after adipocyte transfection for 2 d and 4 d. (F) Protein levels of adipocyte-specific genes, including PPARγ, FASN, and FABP4, analyzed by Western blot. (G) Protein quantification analysis by Western blot. Data are expressed as the mean ± SEM (n = 3). * p < 0.05, ** p < 0.01, vs. NC.