Figure 3.

RNA gel-blot analysis of OsGA2ox1. Ten micrograms of total RNA was loaded per lane. The blot was probed with ³²P-labeled OsGA2ox1 or Os20ox cDNA. The lower part of A and B shows ethidium bromide-stained RNAs corresponding to the above lanes for loading control. A, OsGA2ox1 expression in various organs of wild-type rice. Total RNA was extracted from vegetative shoot apices (Vsa), stems (St), leaf blades (Lb), inflorescence shoot apices (Isa), rachises (Ra), and roots (Rt). B, Effect of GA₃ and uniconazole treatment on the levels of OsGA2ox1 and Os20ox transcripts. Total RNA was extracted from wild-type seedlings treated with 10 μm GA₃ (G) or 10 μm uniconazole (U) or from untreated control plants (C). C, OsGA2ox1 expression in rice dwarf mutants. Total RNA was extracted from wild-type (W), GA-insensitive dwarf mutant (d1), and GA-responsive dwarf mutant (d18) seedlings.